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KMID : 1094020150320020135
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Journal of Veterinary Clinics
2015 Volume.32 No. 2 p.135 ~ p.140
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Effect of trans-10, cis-12 Conjugated Linoleic Acid on Calcium-Dependent Reactive Oxygen Species and Nitric Oxide Production and Nuclear Factor-κB Activation in Lipopolysaccharide-Stimulated RAW 264.7 Cells
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Choi Tae-Won
Kang Byeong-Teck
Kang Ji-Houn
Yang Mhan-Pyo
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Abstract
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Trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) has been shown to participate in the regulation ofanti-inflammatory effects. The objectives of this study were to examine the effects of t10c12-CLA on reactive oxygenspecies (ROS) and nitric oxide (NO) production and nuclear factor-kappaB (NF-κB) activation in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells and to determine whether these effects were associated with change of intracellularcalcium ion (Ca2+). ROS production was increased in LPS-stimulated RAW 264.7 cells, and this effect was suppressedby 1,2-bis-(o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester (BAPTA/AM), a calcium chelator. t10c12-CLA suppressed ROS production in LPS-stimulated RAW 264.7 cells, which was further more decreased bytreatment with BAPTA/AM. These indicated that t10c12-CLA decreases Ca2+-dependent ROS production in LPSstimulatedRAW 264.7 cells. Similarly, NF-κB p65 DNA binding activity and NO production were decreased bytreatment with either t10c12-CLA, BAPTA/AM, or t10c12-CLA and BAPTA/AM combination. However, there wereno differences between t10c12-CLA and BAPTA/AM treatment in NO production of LPS-stimulated RAW 264.7 cells. These data indicate that t10c12-CLA inhibits the increases in ROS and NO production and the NF-κB activation inLPS-stimulated condition. These results suggested that CLA exerts potent anti-inflammatory effects by suppressionof LPS-induced ROS and NO production, and NF-κB activationn via Ca2+-dependent pathway.
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KEYWORD
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conjugated linoleic acid, lipopolysaccharide, reactive oxygen species, nuclear factor-kappaB, nitric oxide, calcium ion
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